yisV [2019-08-28 09:00:22]

similar to transcriptional regulator ([SW|MocR/ GabR family])

locus: BSU_10880

pI: 9.30

mw: 54.11 kDa

protein length: 484 aa

gene length: 1455 bp

function: unknown

product: unknown

essential: no

synonyms: yisW

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.6|Transcription factor/ other/ based on similarity]

Gene

Coordinates: 1,166,737 1,168,191

The protein

Protein family

• [SW|MocR/ GabR family] [Pubmed|22020104]
• [SW|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

Paralogous protein(s)

• [protein|B1A95EAE40AFA40BF12EE9B41287921417A39562|YdfD]

[SW|Domains]

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.

[SW|Cofactors]

• PLP

Structure

• [PDB|1WST] (aminotransferase from Thermococcus profundus, 29% identity)

Expression and Regulation

Operons

• genes: [gene|6A3F08CD939CCDEC1F784D658B815A6D0E1AAC3D|yisV]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B211 (yisW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1210 NBRP B. subtilis, Japan]
• BKE10880 ([gene|6A3F08CD939CCDEC1F784D658B815A6D0E1AAC3D|yisV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCCCCCTTTTTTACAC, downstream forward: _UP4_CGCCTGATGATGTCCTAGGC
• BKK10880 ([gene|6A3F08CD939CCDEC1F784D658B815A6D0E1AAC3D|yisV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCCCCCTTTTTTACAC, downstream forward: _UP4_CGCCTGATGATGTCCTAGGC

References

• 11756427,22020104